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HPSD Media Calculator
Sample - Volume
(ml)
(ml) A
Sample - Cell Concentration
(Mn cells / ml)
(Mn cells / ml) B
per Straw - Volume
(ml)
(ml) C
per Straw - Cell Concentration
(Mn cells / ml)
(Mn cells / ml) D
Volume Used in Sample Testing
(ml)
(ml) O
Standard Processing Volume
E
Total Cells Received in Sample
F = P x B Mn Cells
Proceesing Cell Volume
G = F / E Mn Cells
Step 1 >
1:1 Dilution Volume
(ml)
H = P (ml)
Total Volume After 1:1 Dilution
(ml)
J = H + P (ml)
1:1 Dilution Vol per straw
(ml)
K = (J/F)*D (ml)
Make up Volume Per straw
L = C-K (ml)
Step 2 >
Total Makeup Volume of Media
(ml)
N = L*M
Step 3 >
Total Number of Straws Made
M = F/D (No of Straws)
Available Processing Vol. after QC
P = A-O (ml)
